The Journal of Cell Biology current issueThe Journal of Cell Biology RSS feed -- current issueCHIP promotes Runx2 degradation and negatively regulates osteoblast differentiation- June 16, 2008 Runx2, an essential transactivator for osteoblast differentiation, is tightly regulated at both the transcriptional and posttranslational levels. In this paper, we report that CHIP (C terminus of Hsc70-interacting protein)STUB1 regulates Runx2 protein stability via a ubiquitination-degradation mechanism. CHIP interacts with Runx2 in vitro and in vivo. In the presence of increased Runx2 protein levels, CHIP expression decreases, whereas the expression of other E3 ligases involved in Runx2...http://www.jcb.org/cgi/content/short/181/6/959?rss=1 A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomes- June 16, 2008 Cytoplasmic dynein is the multisubunit motor protein for retrograde movement of diverse cargoes to microtubule minus ends. Here, we investigate the function of dynein variants, defined by different intermediate chain (IC) isoforms, by expressing fluorescent ICs in neuronal cells. Green fluorescent protein (GFP)–IC incorporates into functional dynein complexes that copurify with membranous organelles. In living PC12 cell neurites, GFP–dynein puncta travel in both the anterograde and..http://www.jcb.org/cgi/content/short/181/6/1027?rss=1 |